# **FLASH**: **F**ramework for **LA**rge-**S**cale **H**istomorphometry
# **FLASH**: **F**ramework for **LA**rge-**S**cale **H**istomorphometry
This repository represents a python framework to train, evaluate and apply segmentation networks for renal histological analysis. In particular, we trained an [nnUnet](https://github.com/MIC-DKFZ/nnUNet) for kidney tissue segmentation followed by training another [U-net-like](https://arxiv.org/pdf/1505.04597.pdf) CNN for the segmentation of several renal structures including  tubulus,  glomerulus,  glomerular tuft,  non-tissue background (including veins, renal pelvis),  artery, and  arterial lumen from PAS-stained histopathology data. In our experiments, we utilized human tissue data sampled from different cohorts including inhouse biopsies (UKA_B) and nephrectomies (UKA_N), the *Human BioMolecular Atlas Program* cohort (HuBMAP), the *Kidney Precision Medicine Project* cohort (KPMP), and the *Validation of the Oxford classification of IgA Nephropathy* cohort (VALIGA).
This repository represents a python framework to train, evaluate and apply segmentation networks for renal histological analysis. In particular, we trained an [nnUnet](https://github.com/MIC-DKFZ/nnUNet) for kidney tissue segmentation followed by training another [U-net-like](https://arxiv.org/pdf/1505.04597.pdf) CNN for the segmentation of several renal structures including  tubulus,  glomerulus,  glomerular tuft,  non-tissue background (including veins, renal pelvis),  artery, and  arterial lumen from PAS-stained histopathology data. In our experiments, we utilized human tissue data sampled from different cohorts including inhouse biopsies (AC_B) and nephrectomies (AC_N), the *Human BioMolecular Atlas Program* cohort (HuBMAP), the *Kidney Precision Medicine Project* cohort (KPMP), and the *Validation of the Oxford classification of IgA Nephropathy* cohort (VALIGA).
# Installation
# Installation
1. Clone this repo using [git](https://git-scm.com/book/en/v2/Getting-Started-Installing-Git):<br>
1. Clone this repo using [git](https://git-scm.com/book/en/v2/Getting-Started-Installing-Git):<br>
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@@ -55,9 +55,9 @@ Besides, you can also apply the trained network to our provided exemplary image
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@@ -55,9 +55,9 @@ Besides, you can also apply the trained network to our provided exemplary image